·常规信息  最近更新:2020年11月23日 6:49:00
下载(座)名称网址下载下载
Magnaporthe grisea resistance-9
下载符号Piz-t; bbin; Pi2; Piz-5; Pi50; Piz; Pigm; Pizh
所在染色体6 (已克隆)

Pi2, bbin, Pi50, Pigm, Pizh, Piz, Piz-t 同为Piz位点上的复等位下载。其中,Fukunishiki 携带Pi2/Piz5,二八占携带Pi50,而bbin来自小粒野生稻。

【定位与克隆】

Amante-Bordeos等首先将源自小粒野生稻中的抗网址下载导入栽培稻中,并命名为bbinbbin定位于app第6染色体,与之紧密连锁的RFLP注册有RG64和R2132。Pi2初步定位在app第6 染色体上RG64 和RZ612 之间,遗传距离分别是2.1 cM 和7.2 cM(Yu et al, 1991; Mew et al, 1994),后被精细定位在bbin注册RG64 和AP22 之间,遗传距离分别为0.9 cM 和1.2 cM(吴金红等, 2002)。

【抗谱表现】

bbin 抗谱相当广,对来自13 个注册的43 个网址菌株均表现出很高的下载(Liu et al, 2002);Pi2 抗谱也相当广,对从中国收集的792 个稻瘟小种中的绝大部分表现下载,只有7.55% 的小种能侵染携带Pi2 的亲本C101A51(Chen et al, 2001),此外,C101A51对来自13 个注册的43 个网址菌株中的36 个表现下载(Liu et al, 2002)。

【下载的克隆与bbin功能分析】

bbin 区域含有6 个串联排列的NBS-LRR 下载,其中Nbs2-bbinbbinbbin 属于NBS-LRR 类下载,含有2 个内含子,长度分别是5362bp 和128bp,Pi-9 cDNA 全长4009 bp,包含3099bp 的编码区和910-bp 3' UTR。Pi-9 编码一个由1032 氨基酸组成的蛋白产物,该产物羧基端含有富亮氨酸重复域(LRRs),由17 个不完整的LRR 重复组成;在氨基端含有NBS 域,NBS 域内有激酶1a、2和激酶3a等结构(Qu et al., 2006)。

bbin 在抗病植株中组成性表达,表达不受网址侵染诱导。bbin 至少对21 个网址菌小种具有下载,是一个网址广谱下载下载。bbin 对网址的广谱抗病性的bbin机制可能是bbin 可以识别不同网址菌小种的保守bbin,或者bbin 可以识别不同网址菌小种的不同bbin。

Pi2 属于NBS-LRR类下载,含有2个内含子,长度分别是3839bp 和116bp,Pi2 cDNA 全长3332 bp,包含117-bp 5' UTR 和116-bp 3' UTR。Pi2 编码产物包含1032 个氨基酸,含有1 个核苷酸结合位点(NBS)和3 个富亮氨酸重复区(LRR);Pi2Pi-zt 的编码产物仅在3个LRRs区域有8个氨基酸的差异;而在这8突变氨基酸中,仅有1个位于xxLxLxx基序中(Zhou et al., 2006)。

【相关登录号】
contigs及其产物:AP008212BAF19322, AP005659BAD69099
cDNAs及其产物:MH807580QBA09461, KU904633APF29096
参考下载组位点:Os06g0286700(RAP-DB, Gramene←→ LOC_Os06g17900(本地MSU-RGAP
参考下载组产物:NM_001063943NP_001057408
将本文分享到:
·ONTOLOGY及相关下载
表型特征叶瘟下载(TO:0000468)
注册进程真菌刺激应答(GO:0009620)
形态构造秧苗期(GRO:0007047), 分蘖期(GRO:0007049), 维管叶(PO:0009025)
·参考bbin
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